ELISA Kits
Lablisa® 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit
Catalog No.: LAB13383
| Reactivity | Universal |
| Detection Range | 6.25-400 ng/mL |
| Sensitivity | 1.93 ng/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with 5-HIAA. During the reaction, 5-HIAA in the sample or standard competes with a fixed amount of 5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-HIAA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-HIAA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB13383 |
| Reactivity | Universal |
| Detection Range | 6.25-400 ng/mL |
| Sensitivity | 1.93 ng/mL |
Product Overview
Product name: | Lablisa® 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | 5HIAA |
| Assay Type: | Competitive |
| Sensitivity: | 1.93 ng/mL |
| Standard: | 400 ng/mL |
| Range: | 6.25-400 ng/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 1.5H |
| Research Area: | Metabolic pathway;Tumor immunity;Endocrinology;Urology; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 400 | 0.206 |
| 200 | 0.383 |
| 100 | 0.565 |
| 50 | 0.951 |
| 25 | 1.261 |
| 12.5 | 1.486 |
| 6.25 | 2.07 |
| 0 | 2.565 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant 5-HIAA and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HIAA in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum (n=8) | 92-99% | 96% |
| EDTA plasma (n=8) | 91-101% | 96% |
| Cell culture media (n=8) | 85-94% | 90% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HIAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum (n=8) | 93-100% | 93-103% | 92-100% | 88-95% |
| EDTA plasma (n=8) | 99-105% | 91-99% | 88-95% | 92-98% |
| Cell culture media (n=8) | 93-99% | 90-98% | 87-95% | 88-95% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
Product Images
Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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