ELISA Kits
Lablisa® F-E3(Free Estriol) ELISA Kit
Catalog No.: LAB13324
| Reactivity | Universal |
| Detection Range | 0.63-40 ng/mL |
| Sensitivity | 0.218 ng/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with F-E3. During the reaction, F-E3 in the sample or standard competes with a fixed amount of F-E3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to F-E3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of F-E3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB13324 |
| Reactivity | Universal |
| Detection Range | 0.63-40 ng/mL |
| Sensitivity | 0.218 ng/mL |
Product Overview
Product name: | Lablisa® F-E3(Free Estriol) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | F-E3; Free Estriol |
| Assay Type: | Competitive |
| Sensitivity: | 0.218 ng/mL |
| Standard: | 40 ng/mL |
| Range: | 0.63-40 ng/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 1.5H |
| Research Area: | Endocrinology;Reproductive science;Hormone metabolism; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 40 | 0.232 |
| 20 | 0.333 |
| 10 | 0.608 |
| 5 | 0.894 |
| 2.5 | 1.199 |
| 1.25 | 1.61 |
| 0.63 | 1.909 |
| 0 | 2.702 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant F-E3 and the recovery rates were calculated by comparing the measured value to the expected amount of F-E3 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum (n=8) | 86-96% | 91% |
| EDTA plasma (n=8) | 91-99% | 94% |
| Cell culture media (n=8) | 93-99% | 96% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-E3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum (n=8) | 95-103% | 90-100% | 90-98% | 93-101% |
| EDTA plasma (n=8) | 94-102% | 93-99% | 90-97% | 90-97% |
| Cell culture media (n=8) | 91-100% | 97-104% | 98-104% | 91-99% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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