ELISA Kits
Lablisa® Human α-CTx (Alpha Crosslaps) ELISA Kit
Catalog No.: LAB9306
| Reactivity | Human |
| Detection Range | 93.75-6000pg/mL |
| Sensitivity | 56.25pg/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has
been pre-coated with Human α-CTx. During the reaction, Human α-CTx in the sample or standard
competes with a fixed amount of Human α-CTx on the solid phase supporter for sites on the
Biotinylated Detection Ab specific to Human α-CTx. Excess conjugate and unbound sample or
standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each
micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzymesubstrate
reaction is terminated by the addition of stop solution and the color turns from blue to
yellow. The optical density (OD) is measured spectrophotometric ally at a wavelength of 450 nm. The
concentration of Human α-CTx in tested samples can be calculated by comparing the OD of the
samples to the standard curve.
Technical Specifications
| Catalog Number | LAB9306 |
| Reactivity | Human |
| Detection Range | 93.75-6000pg/mL |
| Sensitivity | 56.25pg/mL |
Product Overview
Product name: | Lablisa® Human α-CTx (Alpha Crosslaps) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | a-CTXI, aCTX-I, A-Cr, Type I Collagen C-Telopeptide-Related Fraction |
| Assay Type: | Competitive |
| Sensitivity: | 56.25pg/mL |
| Standard: | 6000pg/mL |
| Range: | 93.75-6000pg/mL |
| Sample Type: | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL |
| Assay Length: | 3H |
| Research Area: | Tumor immunity; |
Standard curve
| Concentration (pg/mL) | OD |
|---|---|
| 6000 | 0.208 |
| 3000 | 0.372 |
| 1500 | 0.67 |
| 750 | 0.831 |
| 375 | 1.178 |
| 187.5 | 1.627 |
| 93.75 | 1.847 |
| 0 | 2.626 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay
precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Human α-CTx and the recovery rates were calculated by comparing the measured value to the expected amount of Human α-CTx in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 95-102% | 98% |
| EDTA plasma(n=8) | 92-101% | 96% |
| Cell culture media (n=8) | 97-105% | 100% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human α-CTx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 88-97% | 95-103% | 90-98% | 91-98% |
| EDTA plasma(n=8) | 98-105% | 89-97% | 89-98% | 90-96% |
| Cell culture media (n=8) | 92-100% | 95-103% | 97-107% | 91-99% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
Product Images
Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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