ELISA Kits
Lablisa® Human KISS-54(Kisspeptin-54) ELISA Kit
Catalog No.: LAB10696
| Reactivity | Human |
| Detection Range | 62.5-4000pg/mL |
| Sensitivity | 37.5pg/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with Human KISS-54. During the reaction, Human KISS-54 in the sample or standard competes with a fixed amount of Human KISS-54 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human KISS-54. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human KISS-54 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB10696 |
| Reactivity | Human |
| Detection Range | 62.5-4000pg/mL |
| Sensitivity | 37.5pg/mL |
Product Overview
Product name: | Lablisa® Human KISS-54(Kisspeptin-54) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | Metastin, Kisspeptin-54 |
| Assay Type: | Competitive |
| Sensitivity: | 37.5 pg/mL |
| Standard: | 4000pg/mL |
| Range: | 62.5-4000pg/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 1.5H |
| Research Area: | metabolize |
Standard curve
| Concentration (pg/mL) | OD |
|---|---|
| 4000 | 0.225 |
| 2000 | 0.336 |
| 1000 | 0.586 |
| 500 | 0.804 |
| 250 | 1.243 |
| 125 | 1.524 |
| 62.5 | 1.824 |
| 0 | 2.478 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Human KISS-54 and the recovery rates were calculated by comparing the measured value to the expected amount of Human KISS-54 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum (n=8) | 95-103% | 99% |
| EDTA plasma (n=8) | 92-101% | 97% |
| Cell culture media (n=8) | 86-95% | 91% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human KISS-54 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum (n=8) | 92-102% | 92-100% | 93-101% | 92-102% |
| EDTA plasma (n=8) | 90-98% | 97-105% | 97-103% | 94-102% |
| Cell culture media (n=8) | 91-101% | 91-97% | 88-96% | 95-103% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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