ELISA Kits
Lablisa® Rat PACAP-38(Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit
Catalog No.: LAB12911
| Reactivity | Rat |
| Detection Range | 7.82-500pg/mL |
| Sensitivity | 4.69pg/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with Rat PACAP-38. During the reaction, Rat PACAP-38 in the sample or standard competes with a fixed amount of Rat PACAP-38 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PACAP-38. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat PACAP-38 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB12911 |
| Reactivity | Rat |
| Detection Range | 7.82-500pg/mL |
| Sensitivity | 4.69pg/mL |
Product Overview
Product name: | Lablisa® Rat PACAP-38(Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | PACAP-38 |
| Assay Type: | Competitive |
| Sensitivity: | 4.69pg/mL |
| Standard: | 500pg/mL |
| Range: | 7.82-500pg/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 1.5H |
| Research Area: | Epigenetics and Nuclear Signaling; Immunology; Neuroscience |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 500 | 0.215 |
| 250 | 0.397 |
| 125 | 0.591 |
| 62.5 | 0.804 |
| 31.25 | 1.3 |
| 15.63 | 1.533 |
| 7.81 | 1.937 |
| 0 | 2.685 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Rat PACAP-38 and the recovery rates were calculated by comparing the measured value to the expected amount of Rat PACAP-38 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum (n=8) | 93-99% | 96% |
| EDTA plasma (n=8) | 92-100% | 96% |
| Cell culture media (n=8) | 96-103% | 99% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat PACAP-38 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum (n=8) | 96-105% | 91-99% | 96-105% | 90-97% |
| EDTA plasma (n=8) | 92-100% | 88-95% | 94-101% | 97-103% |
| Cell culture media (n=8) | 88-97% | 95-102% | 94-103% | 91-98% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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