Antibody Storage Guide

Antibody Storage & Handling Guide

Proper storage is essential for maintaining antibody activity and specificity.

Storage Guidelines

• Store at -20°C for long-term storage (up to 1 year)
• For short-term use (up to 1 month), store at 4°C
• Avoid repeated freeze-thaw cycles - aliquot and freeze
• Add 50% glycerol for storage at -20°C without freezing
• Keep antibodies in the dark to prevent photodamage
• Do not use sodium azide with HRP-conjugated antibodies

Handling Tips

• Vortex briefly and centrifuge before use
• Use fresh pipette tips to avoid cross-contamination
• Keep antibodies on ice during experiments
• Dilute in appropriate buffer just before use