ELISA Kits
Lablisa® 5-HT(5-Hydroxytryptamine) ELISA Kit
Catalog No.: LAB13387
| Reactivity | Universal |
| Detection Range | 14.07-900 ng/mL |
| Sensitivity | 3.97 ng/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with 5-HT. During the reaction, 5-HT in the sample or standard competes with a fixed amount of 5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB13387 |
| Reactivity | Universal |
| Detection Range | 14.07-900 ng/mL |
| Sensitivity | 3.97 ng/mL |
Product Overview
Product name: | Lablisa® 5-HT(5-Hydroxytryptamine) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | 5HT; Serotonin; N-Methyl-Gamma |
| Assay Type: | Competitive |
| Sensitivity: | 3.97 ng/mL |
| Standard: | 900 ng/mL |
| Range: | 14.07-900 ng/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 1.5H |
| Research Area: | Signal transduction;Infection immunity;Neuro science;Gastroenterology; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 900 | 0.224 |
| 450 | 0.388 |
| 225 | 0.586 |
| 112.5 | 0.9 |
| 56.25 | 1.276 |
| 28.13 | 1.697 |
| 14.06 | 1.991 |
| 0 | 2.753 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant 5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HT in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum (n=8) | 89-95% | 92% |
| EDTA plasma (n=8) | 86-96% | 91% |
| Cell culture media (n=8) | 96-104% | 99% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum (n=8) | 89-96% | 89-98% | 92-99% | 90-98% |
| EDTA plasma (n=8) | 94-102% | 97-105% | 97-104% | 95-103% |
| Cell culture media (n=8) | 89-96% | 96-105% | 94-103% | 97-107% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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