ELISA Kits
Lablisa® Mouse pERK1/2(Phospho Extracellular Signal Regulated Kinase 1/2) ELISA Kit
Catalog No.: LAB12123
| Reactivity | Mouse |
| Detection Range | 1.56-100ng/mL |
| Sensitivity | 0.94 ng/mL |
Description
No description available.
Technical Specifications
| Catalog Number | LAB12123 |
| Reactivity | Mouse |
| Detection Range | 1.56-100ng/mL |
| Sensitivity | 0.94 ng/mL |
Product Overview
Product name: | Lablisa® Mouse pERK1/2(Phospho Extracellular Signal Regulated Kinase 1/2) ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | MAPK3,ERT2,p44-MAPK,MNK1,p44-ERK1,Microtubule-associated protein 2 kinase,Insulin-stimulated MAP2 kinase,MAP kinase isoform p44,Mitogen-activated protein kinase 3,ERK1 |
| Assay Type: | Sandwich |
| Sensitivity: | 0.94 ng/mL |
| Standard: | 100ng/mL |
| Range: | 1.56-100ng/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 3H |
| Research Area: | Signal transduction;Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism; |
Standard curve
| Concentration (pg/mL) | OF | Corrected OD |
|---|---|---|
| 100 | 2.362 | 2.311 |
| 50 | 1.56 | 1.509 |
| 25 | 0.957 | 0.906 |
| 12.5 | 0.525 | 0.474 |
| 6.25 | 0.27 | 0.219 |
| 3.13 | 0.155 | 0.104 |
| 1.57 | 0.094 | 0.043 |
| 0 | 0.051 | 0 |
Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Mouse pERK1/2 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse pERK1/2 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum (n=8) | 82-95% | 88% |
| EDTA plasma (n=8) | 94-107% | 100% |
| Cell culture media (n=8) | 87-95% | 91% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse pERK1/2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum (n=8) | 85-92% | 87-97% | 83-96% | 95-102% |
| EDTA plasma (n=8) | 87-98% | 87-98% | 88-97% | 91-103% |
| Cell culture media (n=8) | 86-92% | 89-97% | 87-98% | 92-105% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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