ELISA Kits
Lablisa® Bovine Ang-II (Angiotensin II) ELISA kit
Catalog No.: LAB13795
| Reactivity | Bovine |
| Detection Range | 31.25-2000pg/mL |
| Sensitivity | 18.75pg/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with Bovine Ang-II. During the reaction, Bovine Ang-II in the sample or standard competes with a fixed amount of Bovine Ang-II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Bovine Ang-II. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Bovine Ang-II in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB13795 |
| Reactivity | Bovine |
| Detection Range | 31.25-2000pg/mL |
| Sensitivity | 18.75pg/mL |
Product Overview
Product name: | Lablisa® Bovine Ang-II (Angiotensin II) ELISA kit |
| Reactivity: | Bovine |
| Alternative Names: | Ang-II; Angiotensin-2 |
| Assay Type: | Competitive |
| Sensitivity: | 18.75pg/mL |
| Standard: | 2000pg/mL |
| Range: | 31.25-2000pg/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 1.5H |
| Research Area: | Enzyme & Kinase; |
Standard curve
| Concentration (μg/mL) | OD |
|---|---|
| 2000 | 0.243 |
| 1000 | 0.37 |
| 500 | 0.685 |
| 250 | 0.803 |
| 125 | 1.203 |
| 62.5 | 1.565 |
| 31.25 | 1.878 |
| 0 | 2.783 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Bovine Ang-II and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine Ang-II in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum (n=8) | 86-95% | 90% |
| EDTA plasma (n=8) | 94-100% | 97% |
| Cell culture media (n=8) | 91-101% | 96% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine Ang-II and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum (n=8) | 88-96% | 91-100% | 87-97% | 98-105% |
| EDTA plasma (n=8) | 89-97% | 87-97% | 91-97% | 99-107% |
| Cell culture media (n=8) | 91-99% | 95-104% | 94-102% | 97-104% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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