ELISA Kits
Lablisa® FastTrack Biotin Quantitative Determination ELISA Kit
Catalog No.: LAB13654
| Reactivity | Universal |
| Detection Range | 7.81-500 pg/mL |
| Sensitivity | 5.35 pg/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with an antibody specific to Biotin. Samples (or Standards) are added to the micro-ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Biotin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Biotin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Biotin. You can calculate the concentration of Biotin in the samples by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB13654 |
| Reactivity | Universal |
| Detection Range | 7.81-500 pg/mL |
| Sensitivity | 5.35 pg/mL |
Product Overview
Product name: | Lablisa® FastTrack Biotin Quantitative Determination ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | Biotin |
| Assay Type: | Sandwich |
| Sensitivity: | 5.35 pg/mL |
| Standard: | 500 pg/mL |
| Range: | 7.81-500 pg/mL |
| Sample Type: | Serum, Plasma and Other biological fluids；Sample Volume=50μL |
| Assay Length: | 0.75H |
| Research Area: | Immunology |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 500 | 0.254 |
| 250 | 0.376 |
| 125 | 0.561 |
| 62.5 | 0.807 |
| 31.25 | 1.089 |
| 15.63 | 1.382 |
| 7.81 | 1.658 |
| 0 | 2.445 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Biotin and the recovery rates were calculated by comparing the measured value to the expected amount of Biotin in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 90-105% | 97% |
| EDTA plasma(n=8) | 80-95% | 87% |
| Cell culture media (n=8) | 83-95% | 89% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Biotin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 80-91% | 89-103% | 82-95% | 86-99% |
| EDTA plasma(n=8) | 86-97% | 86-97% | 90-98% | 80-93% |
| Cell culture media (n=8) | 92-105% | 87-98% | 85-92% | 80-96% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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