Lablisa® FastTrack E2(Estradiol) ELISA Kit

ELISA Kits

Lablisa® FastTrack E2(Estradiol) ELISA Kit

Catalog No.: LAB13655

Competitive

Reactivity	Universal
Detection Range	12.5-800 pg/mL
Sensitivity	7.5 pg/mL

Size:

48T ₹32,550.00 96T ₹42,550.00

Description

This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with an antibody specific to E2. Samples (or Standards) are added to the micro-ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for E2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain E2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of E2. You can calculate the concentration of E2 in the samples by comparing the OD of the samples to the standard curve.

Technical Specifications

Catalog Number	LAB13655
Reactivity	Universal
Detection Range	12.5-800 pg/mL
Sensitivity	7.5 pg/mL

Product Overview

Product name:	Lablisa® FastTrack E2(Estradiol) ELISA Kit
Reactivity:	Universal
Alternative Names:	Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol
Assay Type:	Competitive
Sensitivity:	7.5 pg/mL
Standard:	800 pg/mL
Range:	12.5-800 pg/mL
Sample Type:	Serum, Plasma and Other biological fluids；Sample Volume=50μL
Assay Length:	0.75H
Research Area:	Cytokine;Infection immunity;Immune molecule;

Standard curve

Concentration (ng/mL)	OD
800	0.185
400	0.345
200	0.624
100	0.968
50	1.385
25	1.648
12.5	1.825
0	2.061

Precision

Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.

Matrix	Recovery range	Average
serum(n=8)	83-97%	90%
EDTA plasma(n=8)	80-97%	88%
Cell culture media (n=8)	85-101%	93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.