ELISA Kits
Lablisa® MF-Human IFN-γ (Interferon Gamma) ELISA Kit
Catalog No.: LAB13436
| Reactivity | Human |
| Detection Range | 15.63-1000pg/mL |
| Sensitivity | 9.38 pg/mL |
Description
This ELISA kit uses the Sandwich-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ. Samples (or Standards) are added to the micro-ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IFN-γ. You can calculate the concentration of Human IFN-γ in the samples by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB13436 |
| Reactivity | Human |
| Detection Range | 15.63-1000pg/mL |
| Sensitivity | 9.38 pg/mL |
Product Overview
Product name: | Lablisa® MF-Human IFN-γ (Interferon Gamma) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | IFNG, IFG, IFI, Type II Interferon, IFNγ |
| Assay Type: | Sandwich |
| Sensitivity: | 9.38 pg/mL |
| Standard: | 1000pg/mL |
| Range: | 15.63-1000pg/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 3H |
| Research Area: | Cancer, Immunology |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000 | 3.027 | 2.992 |
| 500 | 2.068 | 2.033 |
| 250 | 1.111 | 1.076 |
| 125 | 0.659 | 0.624 |
| 62.5 | 0.384 | 0.349 |
| 31.25 | 0.208 | 0.173 |
| 15.63 | 0.137 | 0.102 |
| 0 | 0.035 | 0 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Human IFN-γ and the recovery rates were calculated by comparing the measured value to the expected amount of Human IFN-γ in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 87-99% | 93% |
| EDTA plasma(n=8) | 85-99% | 92% |
| Cell culture media (n=8) | 80-92% | 85% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human IFN-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 92-104% | 86-98% | 80-93% | 89-97% |
| EDTA plasma(n=8) | 85-93% | 85-93% | 80-96% | 93-106% |
| Cell culture media (n=8) | 87-99% | 88-98% | 87-103% | 89-97% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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