Lablisa® MF-Mouse OPG (Osteoprotegerin) ELISA Kit

ELISA Kits

Lablisa® MF-Mouse OPG (Osteoprotegerin) ELISA Kit

Catalog No.: LAB13472

Sandwich

Reactivity	Mouse
Detection Range	62.5-4000pg/mL
Sensitivity	37.5 pg/mL

Size:

48T ₹32,550.00 96T ₹42,550.00

Description

This ELISA kit uses the Sandwich-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse OPG. Samples (or Standards) are added to the micro-ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse OPG. You can calculate the concentration of Mouse OPG in the samples by comparing the OD of the samples to the standard curve.

Technical Specifications

Catalog Number	LAB13472
Reactivity	Mouse
Detection Range	62.5-4000pg/mL
Sensitivity	37.5 pg/mL

Product Overview

Product name:	Lablisa® MF-Mouse OPG (Osteoprotegerin) ELISA Kit
Reactivity:	Mouse
Alternative Names:	TNFRSF11B , OCIF, TR1
Assay Type:	Sandwich
Sensitivity:	37.5 pg/mL
Standard:	4000pg/mL
Range:	62.5-4000pg/mL
Sample Type:	Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL
Assay Length:	3H
Research Area:	Signaling transduction, Cancer;

Standard curve

Concentration (pg/mL)	OD	Corrected OD
4000	2.364	2.334
2000	1.524	1.494
1000	0.85	0.82
500	0.45	0.42
250	0.23	0.2
125	0.127	0.097
62.5	0.08	0.05
0	0.03	0

Precision

Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Mouse OPG and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse OPG in samples.

Matrix	Recovery range	Average
serum(n=8)	87-99%	93%
EDTA plasma(n=8)	80-93%	85%
Cell culture media (n=8)	90-105%	97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse OPG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.